Posted by Amisha:
Christie and I worked on the alkaline phoshatase (AP) assay and here’s the data from our first trial. We tested AP activity of Synechocystis sp. PCC6803 cells and spent medium growing at different N:P ratios by measuring p-nitrophenol production from p-nitrophenyl phosphate. Our P-limited cultures (N:P of 53) exhibited the highest level of AP activity (great!), followed by the cultures growing at an N:P of 16. Iron-limited (N:P of 16 with 100 times less Fe than the other cultures) and N-limited (N:P of eight) cultures had very little cellular or extracellular AP activity.