Chemostat Update:

Posted by Amisha:

Christie and I worked on the alkaline phoshatase (AP) assay and here’s the data from our first trial. We tested AP activity of Synechocystis sp. PCC6803 cells and spent medium growing at different N:P ratios by measuring p-nitrophenol production from p-nitrophenyl phosphate. Our P-limited cultures (N:P of 53) exhibited the highest level of AP activity (great!), followed by the cultures growing at an N:P of 16. Iron-limited (N:P of 16 with 100 times less Fe than the other cultures) and N-limited (N:P of eight) cultures had very little cellular or extracellular AP activity.
Presentation1

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One response to “Chemostat Update:

  1. That is very interesting. Thanks for blogging about it. Why do you think the N:P of 16 was higher than the N:P of 16 with lower Fe?
    Also, I have been reading a paper by Frede Thingstad, in L&O Methods, where he monitored APA as he added P. An interesting paper to read as you consider these data.

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